A6012

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A6012

Sigma-Aldrich

 

Aprotinin from bovine lung

saline solution, 5-10 TIU/mL

Synonym:Trypsin inhibitor (basic)
CAS Number:9087-70-1
Linear Formula:C284H432N84O79S7
Molecular Weight:6511.44
MDL number:MFCD00130541

Description

Analysis NoteProtein determined by biuret.
 Another commonly used unit of activity is the KIU (Kallikrein Inhibitor Unit).
1 TIU ∼ 1,300 KIU.
Unit DefinitionOne trypsin inhibitor unit (TIU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmole of N-α-benzoyl-DL-arginine p-nitroanilide (BAPNA) per min at pH 7.8 at 25 °C.
Physical formSolution in 0.9% NaCl and 0.9% benzyl alcohol.
Biochem/physiol ActionsAprotinin is a competitive serine protease inhibitor that inhibits trypsin, chymotrypsin, kallikrein and plasmin. Aprotinin forms stable complexes with and blocks the active sites of enzymes. Binding is reversible with most aprotinin-protease complexes dissociating at pH >10 or <3. Effective concentration equimolar with protease.
General descriptionAprotinin is a protein consisting of 58 amino acids, arranged in a single polypeptide chain that is crosslinked by three disulfide bridges.

Properties

sterilityaseptically filled
formsaline solution
mol wtmol wt ~6,500
concentration5-10 TIU/mL
storage temp.2-8°C

Safety

WGK GermanyNWG

Related Products

Replaced byA6279, Aprotinin from bovine lung

References

referenceDeutscher, M.P., Maintaining protein stability. Meth. Enzymol. 182, 83-89, (1990)
 Dignam, J.D., Preparation of extracts from higher eukaryotes. Meth. Enzymol. 182, 194-203, (1990)
 , Beynon, R.J. and Bond, J.S., ed. Proteolytic Enzymes: A Practical Approach, (1989), 247
 Trautschold, I., et al. Biochem. Pharmacol. 16, 59, (1967)
 Gebhard, W., Review: Biochemistry of aprotinin and aprotinin-like inhibitors. Res. Monogr. Cell Tissue Physiol. 12, 375, (1986)
MerckMerck 13,761